I want to do immunohistochemistry detecting two targets at the same
time. For various reasons I am going to use AEC as substrate for HRP
and BCIP/NBT as AP substrate. Now I am looking for a good
counterstain. Because of the AEC the counterstain has to work with an
aqueous embedding medium.
Has anybody a good idea? Thank you very much for your help in advance,
Am 28.08.2008, 03:06 Uhr, schrieb Bluescript <[Only registered users see links. ]>:
Classical nuclear stains like haematoxilin, methylgreen, nuclear fast red
should work, perhaps you'll have to apply them before immunostaining.
Methylenblue-eosinate (Giemsa, Wright, Papenheim...) would give blue
nuclei and pink cytosol, you'll have to check whether the contrast with
the immunostaining is sufficient. Perhaps switch to
Diaminobenzidine/Nickel for HRP detection (black, chemically quite inert).
But overall, I'd recommend swithching to fluorescence detection, check [Only registered users see links. ]