Transfection in MEF cell lines

AND the problem???????
Jay

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of shashi sharma
Sent: Tuesday, August 26, 2008 10:42 AM
To: [Only registered users see links. ]
Subject: Transfection in MEF cell lines

Dear all
i am suffreing with a serious problem in transfection my drug product
into the MEF cell lines.
Kindly suggest some idea to it.

with regards
Sharma SK

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[ramya313]

Hey!
Did you solve your problem? Cause now I cant transfect MEFs. I just cannot seem to find the write electroporation conditions nor can can I find a good transfection reagent...
help help help!
Regards
Ramya