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Info abt Antibody against Nuclear and cytoplasmic fraction

Info abt Antibody against Nuclear and cytoplasmic fraction - Protocols and Methods Forum

Info abt Antibody against Nuclear and cytoplasmic fraction - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-06-2008, 09:34 AM
Itisam Sarangi
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Default Info abt Antibody against Nuclear and cytoplasmic fraction



hi all,
I recently did a nuclear protein isolation from cultured cells. After
western blot I can see my protein in nuclear and cytoplasmic fractions in
equal concentration.
So just to check the if there is any contamination of cytoplasmic protein in
my nuclear fraction, I am interested to check them aganist an antibody. This
antibody should be against a specific nuclear protein and should be present
in every cell.
I would appreciate any help in this case.
If someone can suggest any antibody that can detect only a cytoplasmic
protein also.

If any one has a good established protocol for nuclear protein isolation
please send me the link.


thanking you for ur help

--
Itisam Sarangi
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