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Anyone has tried a simple vector for Agro mediated Arabidopsis

Anyone has tried a simple vector for Agro mediated Arabidopsis - Protocols and Methods Forum

Anyone has tried a simple vector for Agro mediated Arabidopsis - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 07-16-2008, 05:21 PM
Michael J. Prigge
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Default Anyone has tried a simple vector for Agro mediated Arabidopsis



pBluescript, pUC, pGEM, etc. will not work directly, of course, as
they lack T-DNA border(s) and Agro-compatible origins of
replication. Being a postdoc from a transformation lab, I'm assuming
that you'd know that though. Are you wondering if you can clone these
things into pBluescript, pUC, etc. along with the promoter:reporter?
If so, you probably could, but it'd be a lot easier (and more easily
comparable to others' data) to use any one of the multitude of
existing vectors.
Mike

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