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DNA isolation using Trizol

DNA isolation using Trizol - Protocols and Methods Forum

DNA isolation using Trizol - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 06-11-2008, 02:43 AM
Dunowska, Magda
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Default DNA isolation using Trizol



Hi,
Iíve been trying to isolate RNA and DNA from the same sample (tissue culture fluid spiked with viruses) and, while RNA isolation seems to be workingfine, I donít recover any viral DNA that I originally put into the sample. According to the protocol supplied with Trizol (Invitrogen), DNA should be precipitated by ethanol and pelleted by centrifugation at 2,000 g for 5 minutes, following several washes with the same centrifugation speed to recover the DNA pellet. The recommended speed of the centrifugation seems verylow, so if those of you who use Trizol could tell me whether or not they stick to the recommended protocol, I would be gratefulÖ
Thanks!
Magda


Magda Dunowska, LW, PhD

Senior Lecturer in Veterinary Infectious Diseases (Virology)

Institute of Veterinary, Animal and Biomedical Sciences

Te Kura M‚tauranga Kararehe

Massey University

Palmerston North

New Zealand



Phone : (06) 350-5799 ext 7571

Website : http://ivabs.massey.ac.nz<http://ivabs.massey.ac.nz/>

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