Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

DNA isolation using Trizol

DNA isolation using Trizol - Protocols and Methods Forum

DNA isolation using Trizol - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

LinkBack Thread Tools Display Modes
Old 06-11-2008, 02:43 AM
Dunowska, Magda
Posts: n/a
Default DNA isolation using Trizol

Iíve been trying to isolate RNA and DNA from the same sample (tissue culture fluid spiked with viruses) and, while RNA isolation seems to be workingfine, I donít recover any viral DNA that I originally put into the sample. According to the protocol supplied with Trizol (Invitrogen), DNA should be precipitated by ethanol and pelleted by centrifugation at 2,000 g for 5 minutes, following several washes with the same centrifugation speed to recover the DNA pellet. The recommended speed of the centrifugation seems verylow, so if those of you who use Trizol could tell me whether or not they stick to the recommended protocol, I would be gratefulÖ

Magda Dunowska, LW, PhD

Senior Lecturer in Veterinary Infectious Diseases (Virology)

Institute of Veterinary, Animal and Biomedical Sciences

Te Kura M‚tauranga Kararehe

Massey University

Palmerston North

New Zealand

Phone : (06) 350-5799 ext 7571

Website : http://ivabs.massey.ac.nz<http://ivabs.massey.ac.nz/>

Reply With Quote

dna , isolation , trizol

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Trizol question- color change? Elyse RNA Techniques Forum 0 08-26-2009 09:13 PM
RNA isolation from pigeonpea ravi1709 Protocols and Methods Forum 0 06-01-2009 09:50 AM
RNA Kit v.s. Trizol Method c1bl RNA Techniques Forum 5 02-01-2008 08:28 PM
Problem with extraction of DNA from Trizol max_rempel Epigenetics Forum: DNA Methylation, Histone and Chromatin Study 2 01-11-2008 03:39 PM
23S/16S ratios in RNA preps dependent on isolation method? OBF Protocols and Methods Forum 6 01-27-2004 01:14 AM

All times are GMT. The time now is 05:11 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11117 seconds with 16 queries