Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Proliferation assay in suspension cells

Proliferation assay in suspension cells - Protocols and Methods Forum

Proliferation assay in suspension cells - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-30-2008, 11:21 AM
Kamalian, Laleh
Guest
 
Posts: n/a
Default Proliferation assay in suspension cells



Hi and thanks to everyone who have been helping me so far. I am in trouble again. I need a protocol for proliferation assay in cells that grow in suspension. The method that is used in our lab is MTT assay which is as I underestand for adherent cells.
Thanks Laleh


Reply With Quote
  #2  
Old 06-03-2008, 03:09 PM
Dr Engelbert Buxbaum
Guest
 
Posts: n/a
Default Proliferation assay in suspension cells

Am 30.05.2008, 07:21 Uhr, schrieb Kamalian, Laleh
<[Only registered users see links. ].uk>:


You can also use it for suspension cells, the only problem is that you
have to replace the medium by the MTT solution and the MTT solution by the
propanol/HCl reagent. That is a lot easier with anchored cells where you
simply flick of the solution to be discarded. With suspended cells you'll
have to spin, which is more work. But any other assay you might use
suffers from the same problem.
Reply With Quote
Reply

Tags
assay , cells , proliferation , suspension


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
Characteristics of cancerous cells aftabac Cell Biology and Cell Culture 2 08-26-2011 11:40 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Proliferation assay in suspension cells Kamalian, Laleh Protocols and Methods Forum 0 06-02-2008 08:51 AM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 10:32 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14734 seconds with 16 queries