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#1
05-30-2008, 11:21 AM
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 Proliferation assay in suspension cells Hi and thanks to everyone who have been helping me so far. I am in trouble again. I need a protocol for proliferation assay in cells that grow in suspension. The method that is used in our lab is MTT assay which is as I underestand for adherent cells. Thanks Laleh  |
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#2
06-03-2008, 03:09 PM
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| Proliferation assay in suspension cells Am 30.05.2008, 07:21 Uhr, schrieb Kamalian, Laleh <[Only registered users see links. ].uk>: You can also use it for suspension cells, the only problem is that you have to replace the medium by the MTT solution and the MTT solution by the propanol/HCl reagent. That is a lot easier with anchored cells where you simply flick of the solution to be discarded. With suspended cells you'll have to spin, which is more work. But any other assay you might use suffers from the same problem. |
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| assay , cells , proliferation , suspension |
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