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| Hi, A friend of mince was working with mollusc tissues, and had a similar problem. He had to abandon any DNA extraction kits and went back to phenol-chloroform extraction and ethanol precipitation. As far as I know, this worked for him. Maybe you could try trizol if you also want proteins? Magda Magda Dunowska, LW, PhD Senior Lecturer in Veterinary Infectious Diseases (Virology) Institute of Veterinary, Animal and Biomedical Sciences Te Kura Mātauranga Kararehe Massey University Palmerston North New Zealand Phone : (06) 350-5799 ext 7571 Website : [Only registered users see links. ] -----Original Message----- From: [Only registered users see links. ] [mailto:[Only registered users see links. ].indiana.edu] On Behalf Of kamalaker nasani Sent: Tuesday, 20 May 2008 3:30 p.m. To: [Only registered users see links. ] Subject: DNA EXTRACTION I am working wth okra(Bhendi). As we all know that bhendi will be having lot of mucelage. It became a big problem for DNA extration. And llso for protein extraction for ELISA. Please if anybody have any information related to this please share with me. Thanx in advance -- URS Kamalaker Nasani "The illiterate of the 21st Century will not be those who cannot read or write. The illiterate will be those who cannot learn, unlearn and relearn" -George Bernard Shaw _______________________________________________ Methods mailing list [Only registered users see links. ] [Only registered users see links. ] |
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