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DNA EXTRACTION - Protocols and Methods Forum

DNA EXTRACTION - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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Old 05-21-2008, 12:07 AM
Dunowska, Magda
Posts: n/a

A friend of mince was working with mollusc tissues, and had a similar problem. He had to abandon any DNA extraction kits and went back to phenol-chloroform extraction and ethanol precipitation. As far as I know, this worked for him. Maybe you could try trizol if you also want proteins?

Magda Dunowska, LW, PhD
Senior Lecturer in Veterinary Infectious Diseases (Virology)
Institute of Veterinary, Animal and Biomedical Sciences
Te Kura Mātauranga Kararehe
Massey University
Palmerston North
New Zealand

Phone : (06) 350-5799 ext 7571
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-----Original Message-----
From: [Only registered users see links. ] [mailto:[Only registered users see links. ].indiana.edu] On Behalf Of kamalaker nasani
Sent: Tuesday, 20 May 2008 3:30 p.m.
To: [Only registered users see links. ]

I am working wth okra(Bhendi). As we all know that bhendi will be having
lot of mucelage. It became a big problem for DNA extration. And llso for
protein extraction for ELISA. Please if anybody have any information
related to this please share with me.

Thanx in advance

Kamalaker Nasani

"The illiterate of the 21st Century will not be those
who cannot read or write. The illiterate will be those
who cannot learn, unlearn and relearn"
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