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In situ hybridization with DNA probe

In situ hybridization with DNA probe - Protocols and Methods Forum

In situ hybridization with DNA probe - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-19-2008, 08:26 PM
SONALI MOOKERJEE
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Default In situ hybridization with DNA probe



I am planning to use in situ hybridization to detect gene expression patterns in plant tissue. Most of the protocols I have found use RNA probes although they acknowledge that using RNA probes is more difficult. Does anyone have a protocol for using DNA probes with DIG labelling? Or can anybody point to any publication that describes the technique?

Thanks
Sonali Mookerjee
Michigan State University




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Old 05-24-2008, 12:54 PM
HG
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Default In situ hybridization with DNA probe

hello
It is there in Book InSitu Hybridization A Practical Approach series
(google it u willl get that!!!)
Hope it helps
HG
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dna , hybridization , probe , situ


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