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#1
05-16-2008, 04:56 PM
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 Standard Curve vs DDCT Hi guys, I've recently had some problems getting RNA for some qPCR but my main problem is that I run a standard curve with all my sets of primers to make sure I can compare the efficiency of the reaction properly. With my current RNA yield I don't have enough to run a standard curve. There are differences of opinion in our lab, one person says always run a standard curve to make sure the efficiency is the comparable and one person says you don't need a standard curve just use the controls to compare levels and do Delta  elta CT.Any thoughts? Cheers Yvonne  |
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| curve , ddct , standard |
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