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Standard Curve vs DDCT

Standard Curve vs DDCT - Protocols and Methods Forum

Standard Curve vs DDCT - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-16-2008, 04:56 PM
Yvonne Couch
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Default Standard Curve vs DDCT



Hi guys,

I've recently had some problems getting RNA for some qPCR but my main
problem is that I run a standard curve with all my sets of primers to make
sure I can compare the efficiency of the reaction properly. With my current
RNA yield I don't have enough to run a standard curve. There are
differences of opinion in our lab, one person says always run a standard
curve to make sure the efficiency is the comparable and one person says you
don't need a standard curve just use the controls to compare levels and do
Deltaelta CT.

Any thoughts?

Cheers

Yvonne

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