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#1
05-15-2008, 03:04 PM
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 DNA Solution - Some Queries You can use UV spectrophotometry for determination of ssDNA concentration as follows: at 260 nm, OD value of 1 corresponds to about 40 ug/mL of single-stranded DNA. So from OD at 260 and molecular weight of your oligos you can calculate concentration of your ssDNA in uM: c = OD x 40,000 / M, where M is molecular weight of your oligo in g/mol. You can find many suitable references for this procedure. RM  |
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