Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


DNA Solution - Some Queries

DNA Solution - Some Queries - Protocols and Methods Forum

DNA Solution - Some Queries - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-15-2008, 03:04 PM
Mezencev, Roman
Guest
 
Posts: n/a
Default DNA Solution - Some Queries



You can use UV spectrophotometry for determination of ssDNA concentration as follows:
at 260 nm, OD value of 1 corresponds to about 40 ug/mL of single-stranded DNA. So from OD at 260 and molecular weight of your oligos you can calculate concentration of your ssDNA in uM: c = OD x 40,000 / M, where M is molecular weight of your oligo in g/mol. You can find many suitable references for this procedure.
RM

Reply With Quote
Reply

Tags
dna , queries , solution


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
question about making sodium ortho-vanadate stock solution Z.L. K Protocols and Methods Forum 4 10-20-2009 12:26 PM
Chemistry Acid-Base, Oxidation-Reduction Problems? Jellybean1 Chemistry Forum 0 10-25-2008 10:18 PM
DNA Solution - Some Queries lsl Protocols and Methods Forum 3 05-13-2008 06:24 PM
Cylindrical wave, wave equation, and mistakes h_v_ansari@yahoo.com Physics Forum 2 10-26-2006 01:47 AM
Sci.chem FAQ - Part 6 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:12 AM


All times are GMT. The time now is 10:15 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11999 seconds with 16 queries