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co-transfecting with multiple vectors

co-transfecting with multiple vectors - Protocols and Methods Forum

co-transfecting with multiple vectors - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-05-2008, 06:19 PM
Wendy Grus
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Default co-transfecting with multiple vectors



Hi all-
I am transfecting some HEK 293 T cells to transiently express a few
different genes. Some of these genes are on pcDNA3.1 vectors while some are
on pCI vectors. Will having them on different vectors affect their
expression levels? Will I have to play with the concentrations of each?
Should I put all the genes in one vector (singly)?

Thanks,
Wendy Grus
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