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| Hi all- I am transfecting some HEK 293 T cells to transiently express a few different genes. Some of these genes are on pcDNA3.1 vectors while some are on pCI vectors. Will having them on different vectors affect their expression levels? Will I have to play with the concentrations of each? Should I put all the genes in one vector (singly)? Thanks, Wendy Grus |
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| cotransfecting , multiple , vectors |
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