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ethanol precipitation

ethanol precipitation - Protocols and Methods Forum

ethanol precipitation - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-02-2008, 07:50 PM
yan sun
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Default ethanol precipitation



Dear all,
I did spin label DNA experiment.
I mixed my modified DNA with spin label material in DMF and fomamide
solution. I get 100% label efficiency. (the analytical gel shows that there
is only one spin label DNA band).
I was wondering how to purify my spin labeled DNA? My other site spin
labeled DNAs are not 100% spin label efficiency, so I RUN big PAGE gel, and
use elutor to purified my target spin labeled DNA. during this process, I
lose quite amount material. that is why I don't want to run big gel this
time.
I made a research. looks like ethanol precipitation works.
anyone have experience, please feel free to share.
option 2. can I use freezing dry method?

Thx a lot!!!


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Y. F.S.
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