|Register||Search||Today's Posts||Mark Forums Read|
|Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.|
| ||LinkBack||Thread Tools||Display Modes|
I did spin label DNA experiment.
I mixed my modified DNA with spin label material in DMF and fomamide
solution. I get 100% label efficiency. (the analytical gel shows that there
is only one spin label DNA band).
I was wondering how to purify my spin labeled DNA? My other site spin
labeled DNAs are not 100% spin label efficiency, so I RUN big PAGE gel, and
use elutor to purified my target spin labeled DNA. during this process, I
lose quite amount material. that is why I don't want to run big gel this
I made a research. looks like ethanol precipitation works.
anyone have experience, please feel free to share.
option 2. can I use freezing dry method?
Thx a lot!!!
|ethanol , precipitation|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Ethanol and its history||sunnygirl||Chemistry Forum||2||12-14-2009 08:46 AM|
|Poor yield: DNA purification by phenol/chloroform extraction + ethanol precipitation?||amesy||Protocols and Methods Forum||1||03-27-2009 11:18 PM|
|Difficult Chemistry Problem- ethanol and hydrogen powered SUV?||X_o||Chemistry Forum||0||10-27-2008 07:20 AM|
|Isopropanol vs Ethanol DNA Precipitation||postdock||DNA Techniques||0||02-20-2008 01:33 AM|
|how can I concentrate my plasmid DNA other than using ethanol precipitation||mmn1007||DNA Techniques||0||11-26-2007 09:37 PM|