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#1
05-02-2008, 07:50 PM
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 ethanol precipitation Dear all, I did spin label DNA experiment. I mixed my modified DNA with spin label material in DMF and fomamide solution. I get 100% label efficiency. (the analytical gel shows that there is only one spin label DNA band). I was wondering how to purify my spin labeled DNA? My other site spin labeled DNAs are not 100% spin label efficiency, so I RUN big PAGE gel, and use elutor to purified my target spin labeled DNA. during this process, I lose quite amount material. that is why I don't want to run big gel this time. I made a research. looks like ethanol precipitation works. anyone have experience, please feel free to share. option 2. can I use freezing dry method? Thx a lot!!! -- Y. F.S.  |
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| ethanol , precipitation |
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