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salmon sperm DNA and Northern again

salmon sperm DNA and Northern again - Protocols and Methods Forum

salmon sperm DNA and Northern again - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-01-2008, 05:40 PM
shiri shiri
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Default salmon sperm DNA and Northern again



Hello,

You posted once on a problem you had with your northern hybridization.
As you posted, I experience the same problem, unspecific binding all over
the membrane, even in a blank one. I changed all my compounds, increased the
concentration of the blocking solution but nothing works...
The weird thing it worked great for many years but now no one in our lab,
even ones who were previously successful in it, cannot solve this issue...

I was wondering if you had a chance to solve this problem????

I would appreciate any help/suggestion

THANKS
SHIRI


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