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Page gel

Page gel - Protocols and Methods Forum

Page gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-30-2008, 02:15 PM
Gilbride, Kevin
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Default Page gel



20% is high for acrylamide. Have you considered a tricine gel? If you
absolutely need sds page 4c @ 18mA overnight should work fine. Also, if
you are using a premixed concentrate for your running buffer the pH can
be affected by your water source.
K
-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of yan sun
Sent: Sunday, April 27, 2008 11:39 PM
To: Haviland, David L
Cc: [Only registered users see links. ]
Subject: Re: Page gel


run 20% gel 4w around 10 hours, but the ladder is diffused, I mean the
band is really wide"(
did it happen to you?
if you run under very low watage, it is easy to diffuse.



On 4/27/08, Haviland, David L <[Only registered users see links. ].edu> wrote:
amps
by 9 am
day I
"smile"
always
around
Am I


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Y. F.S.
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