Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

Page gel

Page gel - Protocols and Methods Forum

Page gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

LinkBack Thread Tools Display Modes
Old 04-30-2008, 02:15 PM
Gilbride, Kevin
Posts: n/a
Default Page gel

20% is high for acrylamide. Have you considered a tricine gel? If you
absolutely need sds page 4c @ 18mA overnight should work fine. Also, if
you are using a premixed concentrate for your running buffer the pH can
be affected by your water source.
-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of yan sun
Sent: Sunday, April 27, 2008 11:39 PM
To: Haviland, David L
Cc: [Only registered users see links. ]
Subject: Re: Page gel

run 20% gel 4w around 10 hours, but the ladder is diffused, I mean the
band is really wide"(
did it happen to you?
if you run under very low watage, it is easy to diffuse.

On 4/27/08, Haviland, David L <[Only registered users see links. ].edu> wrote:
by 9 am
day I
Am I

Y. F.S.
Methods mailing list
[Only registered users see links. ]
[Only registered users see links. ]

__________________________________________________ ____________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit [Only registered users see links. ]
__________________________________________________ ____________________

Please consider the environment before printing this e-mail

This email and any files transmitted with it are confidential and
may be legally privileged and are intended solely for the use of
the individual or entity to whom they are addressed. If you are
not the intended recipient please note that any disclosure,
distribution, or copying of this email is strictly prohibited and may
be unlawful. If received in error, please delete this email and any
attachments and confirm this to the sender.

[Only registered users see links. ]

Reply With Quote

gel , page

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Page gel Haviland, David L Protocols and Methods Forum 1 04-27-2008 11:47 PM
Page gel Jayakumar, R Protocols and Methods Forum 0 04-27-2008 07:55 PM
SDS PAGE younes sadramehr Protocols and Methods Forum 2 12-05-2006 07:21 PM
PAGE problem chetanchandra josh Protein Forum 0 11-24-2006 01:44 PM
FS: Old Physics-Related Books Oldbooks78 Physics Forum 0 08-27-2003 02:32 PM

All times are GMT. The time now is 09:54 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11783 seconds with 16 queries