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Page gel

Page gel - Protocols and Methods Forum

Page gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-27-2008, 07:55 PM
Jayakumar, R
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Default Page gel



That is why we have cooooooling circulating water baths to run our
laaaaaaaaaarge SDS PAGE gels.
Jay

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of yan sun
Sent: Saturday, April 26, 2008 7:39 PM
To: [Only registered users see links. ]
Subject: Page gel

Dear all,
I got a question about PAGE electrophoresis gel to confirm.
When we mention that proper volts condition for running a gel is around
20V/cm.
For eample, my gel is 20 cm, which means that proper Volts is ~400V? Am
I right?
If the volts is toooo high, the gel might toooo hot to get good
resolution, right?
Thx a lot!!!!

Frances
--
Y. F.S.
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