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Page gel - Protocols and Methods Forum

Page gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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Old 04-27-2008, 05:14 PM
Wenta Nikola
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Default Page gel

Hi Yan,
you are right; I think you are talking about EMSA or other native gel
systems that use larger gels instead of the Biorad mini gels, right?
We run out gels also at 400 V, but in the cold room. Another possibility
would be water cooling as realized with the Protean ii XL. On the other
hand, you can run that gel at 400 V, but as you mentioned, it would
become hot; therefore, I wouldn't be concerned of the resolution but
more about breakage of glas.

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