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Page gel - Protocols and Methods Forum

Page gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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Old 04-27-2008, 03:56 PM
Haviland, David L
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The reasons you cite is exactly why I always ran my gels on constant amps rather than volts. For a 10% SDS-PAGE I could start it at 5 pm and by 9 am the next morning it was about 3 cm from the bottom. Even during the day I never ran them above 25 mA without some form of cooling else they'd "smile" and make interpretation a bit problematic. In my hands, slower was always better.


-----Original Message-----
From: [Only registered users see links. ] on behalf of yan sun
Sent: Sat 4/26/2008 6:38 PM
To: [Only registered users see links. ]
Subject: Page gel

Dear all,
I got a question about PAGE electrophoresis gel to confirm.
When we mention that proper volts condition for running a gel is around
For eample, my gel is 20 cm, which means that proper Volts is ~400V? Am I
If the volts is toooo high, the gel might toooo hot to get good resolution,
Thx a lot!!!!

Y. F.S.
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Old 04-27-2008, 11:47 PM
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Default Page gel

In article <[Only registered users see links. ].net >, "Haviland, David L" <[Only registered users see links. ].edu> wrote:

That's why I never use large gels to begin with :-)

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