Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Page gel

Page gel - Protocols and Methods Forum

Page gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-27-2008, 03:56 PM
Haviland, David L
Guest
 
Posts: n/a
Default Page gel




The reasons you cite is exactly why I always ran my gels on constant amps rather than volts. For a 10% SDS-PAGE I could start it at 5 pm and by 9 am the next morning it was about 3 cm from the bottom. Even during the day I never ran them above 25 mA without some form of cooling else they'd "smile" and make interpretation a bit problematic. In my hands, slower was always better.

David

-----Original Message-----
From: [Only registered users see links. ] on behalf of yan sun
Sent: Sat 4/26/2008 6:38 PM
To: [Only registered users see links. ]
Subject: Page gel

Dear all,
I got a question about PAGE electrophoresis gel to confirm.
When we mention that proper volts condition for running a gel is around
20V/cm.
For eample, my gel is 20 cm, which means that proper Volts is ~400V? Am I
right?
If the volts is toooo high, the gel might toooo hot to get good resolution,
right?
Thx a lot!!!!

Frances
--
Y. F.S.
_______________________________________________
Methods mailing list
[Only registered users see links. ]
[Only registered users see links. ]


Reply With Quote
  #2  
Old 04-27-2008, 11:47 PM
DK
Guest
 
Posts: n/a
Default Page gel

In article <[Only registered users see links. ].net >, "Haviland, David L" <[Only registered users see links. ].edu> wrote:

That's why I never use large gels to begin with :-)


Reply With Quote
Reply

Tags
gel , page


« Page gel | Page gel »
Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
ssDNA synthesis from PCR and Denaturing PAGE harish DNA Forum 0 10-15-2009 12:56 PM
Page gel Jayakumar, R Protocols and Methods Forum 0 04-27-2008 07:55 PM
SDS PAGE younes sadramehr Protocols and Methods Forum 2 12-05-2006 07:21 PM
PAGE problem chetanchandra josh Protein Forum 0 11-24-2006 01:44 PM
FS: Old Physics-Related Books Oldbooks78 Physics Forum 0 08-27-2003 02:32 PM


All times are GMT. The time now is 05:22 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11702 seconds with 16 queries