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LNA transfection!

LNA transfection! - Protocols and Methods Forum

LNA transfection! - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 04-02-2008, 11:36 AM
Andreas Ploner
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Default LNA transfection!



Hi everyone!
I've got a problem, maybe one of can help me. I want to transfect Locked
nucleic acids (LNAs) into 293T cells. HAs anyone of you expereince with LNA
transfection and whch transfection reagent do you use? Most publication use
Lipofectamin.

At the moment im using Metafectene ([Only registered users see links. ]). At the moment it seems
that knock down somehow works, but I can't find out easily how efficient
LNAs get transfected- so I'd need some transfection reagent to comapre it
to!

Thanks for your answers!

Andi




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  #2  
Old 05-01-2008, 11:35 AM
Niksam
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Default LNA transfection!

On Apr 2, 4:36*pm, "Andreas Ploner" <[Only registered users see links. ].at>
wrote:

Try using invitrogen's RNAiMax or lipofectamine 2000. It produces
better results.

Best,
Nikhil
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  #3  
Old 11-05-2009, 10:38 PM
Pipette Filler
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Default Re: LNA transfection!

Do you still work with LNAs? I will start soon, I'm looking into how much I need to transfect a 96 well plate. Any experience?
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Old 05-07-2010, 08:33 AM
Pipette Filler
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Default Re: LNA transfection!

Hi there

I know this thread is a bit old, but if anyone's still interested in transfecting LNA, you may want to try the Lullaby reagent (OZ Biosciences). See for example the paper by Xu et al., Cell 2009, 137, 1-12 (in hESCs).
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