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#1
03-31-2008, 11:03 PM
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 soft agar transformation assay Hi, could anyone recommend a protocol for a soft agar transfromation assays for mouse embryonic fibroblasts and human diploid fibroblasts? Some technical questions: 1. which agarose do you use (DNA grade?) 2. how do you get the agarase sterile? 3. which medium do you use: 2x DMEM? Your advice is greatly appreciated - thanks! AS  |
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| agar , assay , soft , transformation |
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