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soft agar transformation assay

soft agar transformation assay - Protocols and Methods Forum

soft agar transformation assay - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-31-2008, 11:03 PM
AS
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Default soft agar transformation assay



Hi, could anyone recommend a protocol for a soft agar transfromation
assays for mouse embryonic fibroblasts and human diploid fibroblasts?

Some technical questions:

1. which agarose do you use (DNA grade?)
2. how do you get the agarase sterile?
3. which medium do you use: 2x DMEM?

Your advice is greatly appreciated - thanks!

AS
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