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migration of dyes and DNA thru alkaline agarose gels

migration of dyes and DNA thru alkaline agarose gels - Protocols and Methods Forum

migration of dyes and DNA thru alkaline agarose gels - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-28-2008, 03:36 AM
Sharon
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Default migration of dyes and DNA thru alkaline agarose gels



Dear Experts,

I need to run an alkaline agarose gel to crudely measure the length
of a primer extension reaction (prior to doing a urea gel to closely
and accurately measure length). Does anyone know what the migration
of bromophenol blue and xylene cyanol are in alkaline agarose gels
relative to DNA size (or alkaline polyacrylamide gels)?

Thanks,
Sharon

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agarose , alkaline , dna , dyes , gels , migration


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