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| Hello All, I need help to choose a vector to express a cysteine cross linked dimeric fragment and the monomeric fragment of a protien for NMR structure determination. Since I need to express just a part of a protein what are the limitations (or things to consider) when choosing an expression vector as well as purification of dimeric and monomeric proteins? Which vector(s) may be good candidates? Does choosing a vector depend on the cysteine cross linking procedure? I am wondering whether vector, pEZZ18 (Amersham Bio) can be used to express just a part of a protien as I read that the expression by pEZZ18 is controlled by the lacUV5 and protein A promoters and is not inducible. I am new to molecular cloning and could you please clarify this as well. If you could suggest a common protocol for the expression of cysteine cross linked dimers or provide me with a direction to look for that would be ideal. All your help is greatly appreciated. Thank you very much! Nirosha |
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| crosslinked , cysteine , dimers , expression , find , protein , protocol |
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