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Proliferation Assay (Human T cells)

Proliferation Assay (Human T cells) - Protocols and Methods Forum

Proliferation Assay (Human T cells) - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-03-2008, 05:43 PM
Olivares-Zavaleta, Norma (NIH/NIAID) [F]
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Default Proliferation Assay (Human T cells)



Hi everybody,



I wonder if somebody could share some tips about Proliferation assay
with human cells. I have the protocol for loading the cells with the
CFSE reagent but I'd like to know more details about:



1) How many cells (CFSE loaded) to use?



2) How much time of incubation?



I'll appreciate any comment at this respect.



Norma

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Old 12-11-2010, 05:45 PM
Pipette Filler
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Default Re: Proliferation Assay (Human T cells)

Hi Norma,
I came across your message and I just want to let you know that I do have same problem with CFCE which I wanted to measuring transformation of T cells to compare between normals and patients with immunodeficiency. I would be very thankful if you can send me a protocol you are using.
Thanks.

Faisal ([Only registered users see links. ])
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