I'm a microfluidics researcher trying to develop a bead based
separation method. I have a problem with non-specific binding of
biotinylated beads over a glass slide (forming the base of the
channels) partially coated with streptavidin. Plain glass (slide
cleaned with 1:1 MeOH/HCl for 30 minutes). I have tried the
manufacturers suggestion of running the beads with blocker &
surfactant to prevent binding and it worked fine. I have determined
that its the surfactant that prevents the binding. Priming the channel
with a surfactant solution doesn't help, but in the final application
I cannot have surfactant in the solution. My hope is that there is a
coating that I can apply, either by running a solution down the
channel or by dip-coating the slide, that is compatible with
streptavidin. I have to precoat the slide with streptavidin over a
specific region, so any other coating has to come after.
I know this is sort of a weird question for this forum. So thanks in
advance for all the help.
Dr Matt Davies
Microfluidics and Microengineering Group
University of Hertfordshire
PS resending this as it hasn't been posted yet and thought maybe it
was because the first one wasn't in plain text format.