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What smear bands mean?

What smear bands mean? - Protocols and Methods Forum

What smear bands mean? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 02-26-2008, 09:56 AM
TRAN HO QUANG
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Default What smear bands mean?



What smear bands mean for above and below main band of DNA and PCR products?. Which one corespondent for A260, A260/A280 or A230 ratio?





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  #2  
Old 02-26-2008, 11:47 PM
DK
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Default What smear bands mean?

In article <[Only registered users see links. ].net >, TRAN HO QUANG <[Only registered users see links. ]> wrote:

They mean other DNA molecules - although the smear above may
mean using bad agarose and/or overloading the gel.


All of them. Though I am not sure about 230 - I'd imagine it's
pretty useless regardless. (Someone please educate me if
that's not the case).

DK


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  #3  
Old 02-27-2008, 04:09 AM
Sudheendra Rao N R
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Default What smear bands mean?

Hi,
well, i know (i think i know ) about the ratios
260/280: DNA purity (ideal more than 1.6)
DNA max at 260 (i guess its because of Adenine)
RNA max at 280 (??Uridine)
260/230: i guess organic solvents have absorption maxima at 230nm..like
ethanol, phenol..isopropanol..
whats this smear band???

On Wed, Feb 27, 2008 at 5:17 AM, DK <dk@no.email.thankstospam.net> wrote:




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  #4  
Old 02-27-2008, 10:03 PM
AK
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Default What smear bands mean?

260/280 between 1.80-1.90 together with 260/230 around 2.0 indicates pure
plasmid DNA.

260/280 > 2.0 = too much RNA in sample

260/280 < 1.8 = protein contamination

Regards,
AK


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