Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:
- NEW: small activating RNA (saRNA)
- update: RNA intereference and siRNA
- update: microRNA
- update: HRM (High Resolution Melt)
- qPCR application workshops in 2008
small activating RNA ( saRNA or RNAa )
[Only registered users see links. ]
Small double-stranded RNA (dsRNA) has been found to silence gene
expression by an evolutionally conserved mechanism known as RNA
interference or RNAi. Such dsRNAs are called small interfering RNAs or
siRNA. RNAi can occur at both transcriptional and post-transcriptional
levels. Surprisingly, various recent studies (see below) have found
that dsRNA can also activate gene expression, a mechanism that has
been termed "small RNA-induced gene activation" or "saRNA" or "RNAa".
It has been shown that dsRNAs targeting gene promoters induce potent
transcriptional activation of associated genes. Both studies
demonstrate RNAa in human cells using synthetic dsRNAs termed small
activating RNAs (saRNAs). Endogenous microRNAs (miRNA) that cause RNAa
has also been found in humans. It is currently unknown if RNAa is
conserved in other organisms.
- Small dsRNAs induce transcriptional activation in human cells.
- A small modulatory dsRNA specifies the fate of adult neural stem
- Activating gene expression in mammalian cells with promoter-targeted
- Transcriptional activation by small RNA duplexes.
- Regulation of endothelial nitric oxide synthase by small RNA.
- RNA interference: hitting the ON switch.
- When microRNAs acivate translation.
- Switching from repression to activation: microRNAs can up-regulate
- Activation of an oncogenic microRNA cistron by provirus integration.
- Silencing of microRNAs in vivo with 'antagomirs'.
- Specificity, duplex degradation and subcellular localization of
HRM is a novel, homogeneous, close-tube, post-PCR method, enabling
genomic researchers to analyze genetic variations (SNPs, mutations,
methylations) in PCR amplicons. It goes beyond the power of classical
melting curve analysis by allowing to study the thermal denaturation
of a double-stranded DNA in much more detail and with much higher
information yield than ever before. HRM characterizes nucleic acid
samples based on their disassociation (melting) behavior. Samples can
be discriminated according to their sequence, length, GC content or
strand complementarity. Even single base changes such as SNPs (single
nucleotide polymorphisms) can be readily identified. The most
important High Resolution Melting application is gene scanning - the
search for the presence of unknown variations in PCR amplicons prior
to or as an alternative to sequencing. Mutations in PCR products are
detectable by High Resolution Melting because they change the shape of
DNA melting curves. A combination of new-generation DNA dyes, high-end
instrumentation and sophisticated analysis software allows to detect
these changes and to derive information about the underlying sequence
With the new qPCR INFO PORTAL and all the presented tools we will help
you with to find the right information about qPCR and related topics
in Molecular Biology in the literature and in the World Wide Web.
=> Papers / Protocols / Methods / Databases / Alets / Feeds / Books /
Forums / E-mail / Directory
At the TATAA Biocenter Germany we offer qPCR application workshops,
the 3-day Core Module and a 2-day Biostatistics Module. qPCR courses
are held in regularly in Göteborg, Sweden, in English and in Freising-
Weihenstephan, Germany, in German and English, and in Prague, Czech
Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and specialized
trainings will be held as well across Europe and world-wide. TATAA
Biocenter Germany courses are held in cooperation with the Institute
of Physiology, located at the Technical University of Munich, in
Freising-Weihenstephan, near Munich, very close to the Munich Airport
(MUC). For more information and to register for the qPCR application
workshops, please see our web page: [Only registered users see links. ]
* 3rd - 7th March 2008 (in Freising, Germany, English language)
* 5 - 9th May 2008 (in Freising, Germany, Kurs wird in DEUTSCH
gehalten, German language)
* 7 - 11th July 2008 (in Freising, Germany, English language)
Please register here => [Only registered users see links. ]