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Lipo2000 leads to cell apoptosis?

Lipo2000 leads to cell apoptosis? - Protocols and Methods Forum

Lipo2000 leads to cell apoptosis? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 02-20-2008, 05:29 AM
WANG XF
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Default Lipo2000 leads to cell apoptosis?



Usually transfection was completed with lipofectamine 2000 in our lab. I am
detecting apoptosis induced with H2O2, and after transfection of the
apoptosis sensor construct into cells, apoptosis could be found in the
absense of H2O2.
lipofectamine 2000 or other transfection reagents based on liposome will
lead to cell apoptosis?

Thanks.

LW
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  #2  
Old 02-21-2008, 01:14 AM
DK
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Default Lipo2000 leads to cell apoptosis?

In article <[Only registered users see links. ].net >, "WANG XF" <[Only registered users see links. ]> wrote:

Every lipofection agent I tried was cytotoxic and, based on appearance
of cells after transfection, seemed to have induced apoptosis in a
proportion of cells. I also believe cytotoxicity of most/all lipofection
reagents is a well-known fact.

DK
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  #3  
Old 02-21-2008, 04:52 AM
Kyle Legate
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Default Lipo2000 leads to cell apoptosis?

WANG XF wrote:
Sure it will. The transfection protocol calls for cells to be >90%
confluent at the time of transfection. If breakdown of the nuclear
envelope concomitant with cell division is required to allow the DNA to
enter the nucleus then you are counting on apoptosis to make your
experiment work.
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