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Inducer of apoptosis in animal

Inducer of apoptosis in animal - Protocols and Methods Forum

Inducer of apoptosis in animal - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 02-19-2008, 09:41 AM
wxfhome@gmail.com
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Default Inducer of apoptosis in animal



Hello!

I want to test a method which can detect apoptosis in mice, and I do
not know how to induce apoptosis in mice. Could anyone give some
suggestions about the inducer and protocol? I see H2O2 can induce
apoptosis in cell culture.

Thank you all.

LW
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  #2  
Old 02-19-2008, 06:19 PM
Kyle Legate
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Default Inducer of apoptosis in animal

[Only registered users see links. ] wrote:
I'm not sure that inducing apoptosis in a whole mouse would pass ethics
boards. I know they wouldn't see it as necessary. Select a tissue which
is known to have a significant amount of natural apoptosis, like the
skin during hair follicle catagen (around 8 weeks after birth) or the
mammary gland sometime after weaning, during involution, and test your
method on those samples.
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  #3  
Old 02-19-2008, 06:47 PM
Pow Joshi
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Default Inducer of apoptosis in animal

On 19/02/2008, [Only registered users see links. ] <[Only registered users see links. ]> wrote:



hello, you mean you want to induce apoptosis in vivo? Does it have a
specific region of an organ system? Because, an apoptosed mouse, I'd
imagine is a very dead mouse.

Pow

Thank you all.
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  #4  
Old 02-19-2008, 10:07 PM
WS
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Default Inducer of apoptosis in animal



Yes, very dead indeed. In German, this is called "mausetot" (as dead
as a mouse...)

What about taking out the organs of interest, perfusing them like in
the Langendorf Heart Model and include apoptosis inducers in the
buffer? As lethal to mice as whole animal apoptosis, but a bit more
human...

Best,

Wo
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  #5  
Old 02-20-2008, 05:01 AM
WANG XF
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Default Inducer of apoptosis in animal

Hi,

Thanks a lot for your help!

Yes, I want to induce and observe apoptosis in vivo, and the apoptosis
should take place at some tissues or regions (subcutaneous or liver, et
al.), but not the whole mouse.

LW


On 2/20/08, Pow Joshi <[Only registered users see links. ]> wrote:
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