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wxfhome@gmail.com 02-19-2008 09:41 AM

Inducer of apoptosis in animal
 
Hello!

I want to test a method which can detect apoptosis in mice, and I do
not know how to induce apoptosis in mice. Could anyone give some
suggestions about the inducer and protocol? I see H2O2 can induce
apoptosis in cell culture.

Thank you all.

LW

Kyle Legate 02-19-2008 06:19 PM

Inducer of apoptosis in animal
 
[Only registered and activated users can see links. Click Here To Register...] wrote:
I'm not sure that inducing apoptosis in a whole mouse would pass ethics
boards. I know they wouldn't see it as necessary. Select a tissue which
is known to have a significant amount of natural apoptosis, like the
skin during hair follicle catagen (around 8 weeks after birth) or the
mammary gland sometime after weaning, during involution, and test your
method on those samples.

Pow Joshi 02-19-2008 06:47 PM

Inducer of apoptosis in animal
 
On 19/02/2008, [Only registered and activated users can see links. Click Here To Register...] <[Only registered and activated users can see links. Click Here To Register...]> wrote:



hello, you mean you want to induce apoptosis in vivo? Does it have a
specific region of an organ system? Because, an apoptosed mouse, I'd
imagine is a very dead mouse.

Pow

Thank you all.

WS 02-19-2008 10:07 PM

Inducer of apoptosis in animal
 


Yes, very dead indeed. In German, this is called "mausetot" (as dead
as a mouse...)

What about taking out the organs of interest, perfusing them like in
the Langendorf Heart Model and include apoptosis inducers in the
buffer? As lethal to mice as whole animal apoptosis, but a bit more
human...

Best,

Wo

WANG XF 02-20-2008 05:01 AM

Inducer of apoptosis in animal
 
Hi,

Thanks a lot for your help!

Yes, I want to induce and observe apoptosis in vivo, and the apoptosis
should take place at some tissues or regions (subcutaneous or liver, et
al.), but not the whole mouse.

LW


On 2/20/08, Pow Joshi <[Only registered and activated users can see links. Click Here To Register...]> wrote:


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