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site directed mutagensis (Jose de las Heras)

site directed mutagensis (Jose de las Heras) - Protocols and Methods Forum

site directed mutagensis (Jose de las Heras) - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 02-17-2008, 05:52 PM
Wenta Nikola
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Default site directed mutagensis (Jose de las Heras)



Hello!
In our lab, we use Stratagene's Pfu Turbo with up to 14 mins elongation time (annealing temoerature is important!). Then digest with DpnI and transformation into XL2-Blue. Works fine!
Cheers,
Niko

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