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Qiagen high speed maxi

Qiagen high speed maxi - Protocols and Methods Forum

Qiagen high speed maxi - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 02-15-2008, 06:56 PM
Steve Crampton
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Default Qiagen high speed maxi



We have been using the Qiagen high speed maxi prep kit to isolate
plasmid DNA for a while and have had some problems. We have
standardized our growth time and pretty much always prep high copy
plasmids such as PcDNA. The problem is that sometimes we get 1 mg of
DNA and sometimes we get no DNA at all! My wife (in a separate lab)
has also had this problem and they did a side by side comparison (2
different columns, same cultures, etc) and one had 0.8mg and the other
no DNA. Has anyone else had a similar yield problem with this kit?
Steve Crampton

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  #2  
Old 02-16-2008, 02:37 AM
DK
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Default Qiagen high speed maxi

In article <[Only registered users see links. ].net >, Steve Crampton <[Only registered users see links. ]> wrote:

That's pretty odd. Of all things, the columns would be the last
thing I'd blame. You can be 99.999% sure they are packed with
the same ion exchanger!

Is it possible some of them are not wetted properly? If I suspected
this being the case, I'd wash the column with ethanol and then water
before proceeding with the protocol.

Personally, I've never experienced any yield problems with
Qiagen's Maxi preps.

DK
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  #3  
Old 02-16-2008, 05:47 AM
Sudheendra Rao N R
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Default Qiagen high speed maxi

hi,
check for the common lot number???
the dna yeild from qiagen columns are not going to be same from every
column..however they will surely not differ drastically.
i have used QIA100, 500 but not 1000.

best,
sudheendra.

On Sat, Feb 16, 2008 at 8:07 AM, DK <[Only registered users see links. ]> wrote:




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