SyBR green home mix (Duncan Clark)
We use our own QPCR MM that includes the SyBR green from Molecular
probes. This home made mix is much cheaper than premade mixes from
most companies. the reference for the mix is below the recipe. Of
course, you still have to get the Hot start taq (we use Qiagens).
Quantitative PCR Master Mix (2X)
makes 10mL store in aliquots in the -20
Stock reagent Volume
1M Tris pH 8.3 (UV irradiated)
25mM MgCL2 (Qiagen)
10mg/mL BSA Omnipure Fraction V
10mM dNTP Mix
100X SyBR Green 66.67
dH2O (irradiated DEPC) 5.032 mL
**for use: Add 10μL Qiagen HotStarTaq per 1mL Master Mix
2μL upper primer
2μL lower primer
Reference for MM
Phosphoinositide 3-kinase and Bruton's tyrosine kinase regulate
overlapping sets of genes in B lymphocytes.
Fruman DA, Ferl GZ, An SS, Donahue AC, Satterthwaite AB, Witte ON
Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):359-64
Re: SyBR green home mix (Duncan Clark)
I'm interested in seeing whether anyone has tried the SyBr green home mix in this post. We've being using IQ SYBR GRN SUPERMIX from Bio-Rad and it's pricy. Also, in the recipe the concentration of SyBR Green is 100x, I wonder what does this mean? Can you buy SYBR Green I from Molecular Probes that says 100x in concentration?
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