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what is the most quantitative method for SNP detection

what is the most quantitative method for SNP detection - Protocols and Methods Forum

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Old 02-14-2008, 01:14 PM
Zhong Silin
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Default what is the most quantitative method for SNP detection



Hi everyone

If I have a mixture of 2 DNA, which has just one SNP, say 40% GGG and 60% GTG.
What SNP detection method is the best to quantify it?

Is the Beckman SNP detection method the best? I heard that their kit is similar to a dye terminator sequence reaction. The reaction doesn't contain normal dNTP. So only 1 bp can be extended and therefore the primer will link to one dye-ddNTP. Maybe this will generate less background than a normal sequencing?

Is there a better way?

Thanks in advance
silin

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