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| Hi everyone If I have a mixture of 2 DNA, which has just one SNP, say 40% GGG and 60% GTG. What SNP detection method is the best to quantify it? Is the Beckman SNP detection method the best? I heard that their kit is similar to a dye terminator sequence reaction. The reaction doesn't contain normal dNTP. So only 1 bp can be extended and therefore the primer will link to one dye-ddNTP. Maybe this will generate less background than a normal sequencing? Is there a better way? Thanks in advance silin This message has been checked for viruses but the contents of an attachment may still contain software viruses, which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. |
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| detection , method , quantitative , snp |
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