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Media left at 37 C O/N?

Media left at 37 C O/N? - Protocols and Methods Forum

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Old 02-10-2008, 08:15 PM
Kamalian, Laleh
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Default Media left at 37 C O/N?

We are usually advised not to leave the media in the water bath for long. However considering we grow our cells in the same media at 37' it shoudnt'treally do much damage to the media. The only thing is that if you are using antibiotics in your media (like pen/strep) they degrade after 3 days at 37' and this is one of reasons we have to change the media after 2-3 days.

From: Tim Eggleston
Sent: Fri 08/02/2008 02:58
To: [Only registered users see links. ]
Subject: Media left at 37 C O/N?

The question regarding heating the RPMI media to 60 C brought to mind
a question I've wondered about from time to time. If media, such as
DMEM or MEM, is left in a 37 C water bath O/N, will be ok to use it
the next day. I have always advised people to pitch it due to the
possible degradation of L-glutamine, but I have always wondered how
much degradation actually occurs, if any. Thanks!

Tim Eggleston
Horne Lab
Research Assistant
2-505 Bowen Science Building
University of Iowa

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