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R: buffer preparation

R: buffer preparation - Protocols and Methods Forum

R: buffer preparation - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 02-06-2008, 03:02 PM
Prof. Piero Sestili
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Default R: buffer preparation



First prepare a 2 M solution by diluting 2.26 ml of your stock to 10 ml,
then make serial 1:10 dilutions down to 2 mM
or
prepare a 200 mM solution first diluting 226 ul of your stock to 10 ml
and then another 1:100 dilution to 2 mM. As you prefer....


Piero


Prof. Piero Sestili
Istituto di Farmacologia e Farmacognosia e
Centro di Ricerca sull'Attività Motoria
Università degli Studi di Urbino "Carlo Bo"
Via "I Maggetti" 26
61029 URBINO (PU)
Tel. 0722 303414; 0722 305524
Fax 0722 303401


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Inviato: mercoledì 6 febbraio 2008 12.11
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Oggetto: buffer preparation

Hello,

How do i work this out?
:

I need 2mM H2O2 from a stock solution H2O2 of 30%, density 1.11 gr/ml,
M.W. 34


anybody?
thanks upfront

b
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