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hydrophobic protein extraction

hydrophobic protein extraction - Protocols and Methods Forum

hydrophobic protein extraction - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 01-29-2008, 06:17 PM
James Munro
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Default hydrophobic protein extraction



I am trying to extract a hydrophobic 20kDa protein from feed which contains oils. Has anyone got a good method or suggestions how to separate the protein from the oil so I can detect the protein in a ELISA?

Thanks
James
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  #2  
Old 07-09-2011, 08:46 PM
Pipette Filler
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Default Re: hydrophobic protein extraction

I think you can use reverse phase catridge to extract hydrophobic protein out.since its resin has 18 carbons in it.whereby when pour your sample the hydrophobic protein will binds with the carbons then u can eluate them using solvents.
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Old 12-08-2011, 10:23 AM
Pipette Filler
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Default Re: hydrophobic protein extraction

I agree. That has worked for me before.
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