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Calculating Protein Concentration

Calculating Protein Concentration - Protocols and Methods Forum

Calculating Protein Concentration - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #11  
Old 01-24-2008, 09:05 PM
Jose de las Heras
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"Yvonne Couch" <yvonne.couch@dpag.ox.ac.uk> wrote in message
news:mailman.245.1200935329.2451.methods@net.bio.n et...

???

nobody in the lab knew the answer???

that's very worrying, if true.


You are right.

It was a basic question. I remember looking at what molecules, Avogadro,
moles etc in high school. A simple concept that once grasped, it stays.
Okay, maybe some people have to wait till their undergrad years and re-learn
there... I'm surprised somebody is able to pass their basic chemistry s an
undergrad and not grasp these concepts... But at teh end of the day, I'd
rather have someone asking a question than not.
It is not right to patronise you for asking a question like this. But I
don't think you were exactly patronised. A number of people have expressed
surprise at how somebody at the level you are didn't know this. I don't know
you, but you are probably a reasonably intelligent person. So a question
like this it's surprising... but that's all.
Some people will treat the ignorance of others to putthem down, tomake them
beel bigger. Ignore them. You'll come across them. They cannot be avoided...
but one grows a thick skin, and learns who to pay attention to and who to
ignore... and even arrogant bastards have sometimes something useful and
interesting to say. In other words, scientists are just human, with the same
defects as the population at large.

I suppose that generating a thread like this might make one feel a bit
"shy", and put off.
Don't let it get to you. It's not a big deal. Take the useful information,
ignore teh rest. Actually, it's just like reading papers... you don't just
read what's written and accept it... you read it critically and judge
whether the conclusions of teh authors truly reflect what the experiments
demonstrate. You accept some, and some you put in the "hmmm, not fully
convinced yet, but thanks" bag. YOU decide. You have to think for yourself,
which is not the same as being isolated and not communicating. Ask any
question, by all means, but it's usually a good habit to think a bit about
it by yourself, if you can. You learn more that way. But you always learn
more by asking than by not aking... if somebody sneers... that's their
problem, and you are solving yours.

ok, I'll stop now

Jose



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  #12  
Old 01-24-2008, 09:23 PM
Dr Engelbert Buxbaum
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Am 22.01.2008, 14:19 Uhr, schrieb Tom Anderson <[Only registered users see links. ].uk>:



The most important thing is to do a Postdoc abroad. Ideal would be to do
it in a country with a language different from your native one. However,
since many native English speakers never bothered to master another
language they have to take the second best solution to go to another
English speaking country with at least a different culture. And GB and US
are countries separated by their common language.
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  #13  
Old 01-25-2008, 12:57 AM
Han
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"Dr Engelbert Buxbaum" <engelbert_buxbaum@hotmail.com> wrote in
newsp.t5gdpu0266vu6s@bengelbert-dm.rusm.rossu.loc:


Yes, the verb "to table" means opposite things in British and American
English.

As a biochemistry major in Utrecht, I worked in a lipid biochemistry lab.
In Van Deenen's lab the day to day language was of course Dutch, but
presentations by "native" as well as foreign investigators were always in
"English", be the presenter British, French, Portuguese, Polish, German,
or American. Sometimes the verbal parts went faster, sometimes slower.

The scientific part of the language was universal.

--
Best regards
Han
email address is invalid
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  #14  
Old 01-25-2008, 07:25 PM
Tom Anderson
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On Thu, 24 Jan 2008, Dr Engelbert Buxbaum wrote:


That's exactly my situation.


Yes. I'm hoping to go to the Netherlands.



tom

PS Actually, i did once speak French pretty well, but that was a long time
ago. I don't know how quickly i could pick it up again. In any case,
getting a job in France seems to be hard enough for French people ...

--
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) [Only registered users see links. ]

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