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how to calculate Kcat value of enzyme

how to calculate Kcat value of enzyme - Protocols and Methods Forum

how to calculate Kcat value of enzyme - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 01-21-2008, 02:35 PM
Analia Alet
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Default how to calculate Kcat value of enzyme



Hi, kcat = Vmax/Km, where Vmax could be expressed in mnol/(mg min) and Km in
uM, so kcat is expressed in min-1

one good book explaining all this is

Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State
Enzyme Systems (Wiley Classics Library)by Irwin H. Segel (Author)

Best Regards
Analía

Good evening

From your data you can first calcualte the molar amount of enzyme per
reaction. Then re-express the velocity as mmole/min/mmole = 1/min, convert
min to sec and you have the turnover number in 1/s = kat. If you have
problems with any of that, ask your instructor to go through the
calculation with you.


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