| | Re: Stable transfection in suspension cells
HI, every one
I am new in the station. I actually intend to make some stable cell line using serum-free suspension cells .
Anyone knows if the proposed carboxymethlycellulose method could work in serum-free suspension culture? Could carboxymethylcellulose slow down cell growth as these cells are normally culture in serum_free conditions?
Could anyone forward the protocol to use carboxymethylcellulose for identification of clones to me?
Thank you in advance