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Stable transfection in suspension cells

Stable transfection in suspension cells - Protocols and Methods Forum

Stable transfection in suspension cells - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #11  
Old 10-02-2011, 01:19 AM
Pipette Filler
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Default Re: Can you heat RPMI 1640?

Hi
I could not understand how do you prepare 10X methylcellulose. Does not the solution again solidify when it is brought to room temperature?

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Old 10-03-2011, 02:29 AM
Pipette Filler
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Cool Re: Stable transfection in suspension cells

I tell you. Even since I posted this question, no one has ever given me the protocol or answered my question. So I don't think the first one asked for the protocol ever got it.
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Old 03-01-2012, 12:47 PM
GMB GMB is offline
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Default Re: Stable transfection in suspension cells

HI, every one

I am new in the station. I actually intend to make some stable cell line using serum-free suspension cells .


Anyone knows if the proposed carboxymethlycellulose method could work in serum-free suspension culture? Could carboxymethylcellulose slow down cell growth as these cells are normally culture in serum_free conditions?

Could anyone forward the protocol to use carboxymethylcellulose for identification of clones to me?

Thank you in advance
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