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Calculating Protein Concentration

Calculating Protein Concentration - Protocols and Methods Forum

Calculating Protein Concentration - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 01-18-2008, 10:08 PM
Terran
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Default Calculating Protein Concentration



I too thought this question seemed so silly, and to investigate the
simplicity of the answer:



I searched "Dalton definition" at [Only registered users see links. ] <http://www.google.com/>



Looking for an .edu site I found:



Dalton = measure of molecular weight or mass. One hydrogen atom has mass of
1 Da. Proteins and other macromolecule molecular weights are usually
measured in kDa or kD (kilodaltons) - 1000 Da. From
([Only registered users see links. ] <http://www.biochem.northwestern.edu/>
)



I didn't know the exact answer in the beginning, but from the definition it
is a simple conversion problem.



Freshmen with general chemistry could solve this. Simple laziness.



~Terran



Graduate student

Chemical and Biological Engineering Dept

South Dakota School of Mines and Technology

Rapid City, SD 57701



-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of WS
Sent: Friday, January 18, 2008 12:45 PM
To: [Only registered users see links. ]
Subject: Re: Calculating Protein Concentration



Dear AK,



seems that even at famous Oxford either the students or the teaching

have become worse...



Actually, this stuff is a sort of "first day, first lesson's

knowledge" in

almost any subject related to natural science. More to worry, a PhD

(!) student in my lab asked me exactly the same question a few days

ago, too. The funny thing is, when I told him to treat the problem the

same way as to make a sodium chloride solution, he could solve the

problem instantly.



What makes me especially worry is that this kind of questions in this

NG constantly seems to increase. I do not think it just lazyness, as

for a newbie to find this NG on the web, usually, you need to have

done some googling before on behalf of your actual problem. My

impression is that many of those girls&guys really don't know how to

solve their problems and somehow manage to discover the bionet.



Hey students, please comment on this, your opinion and experience is

wanted here!!! Did nobody teach you these things, do you not know

where to find this kind of textbooks and lessens or are you really

just lazy?



Hey experts, is there any book/website/pdf/etc like {Mathematics,

Chemistry, Logical Thinking, Statistics, Planning Experiments and

Evaluating Experimental Data} in {Biochemistry, Molecular Biology,

Life Science} for Dummies?



Any ideas?



Wo





On 18 Jan., 18:40, "AK" <[Only registered users see links. ]> wrote:

since








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  #2  
Old 01-21-2008, 09:04 AM
StewJW
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Default Calculating Protein Concentration

Its a forum and so it shouldn't matter how basic is the question.
Often its only when you start applying material you've learnt as an
undergrad. that you begin to understand it.
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  #3  
Old 01-21-2008, 09:08 AM
StewJW
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Default Calculating Protein Concentration

I should add, I've known a lot of Biologists who still have difficulty
understanding the concept of the mole very late in their careers.
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  #4  
Old 01-21-2008, 01:43 PM
DK
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Default Calculating Protein Concentration

In article <[Only registered users see links. ]>, StewJW <[Only registered users see links. ]> wrote:

One wonders how they managed to:

- graduate from high school;
- graduate from college;
- obtain an advanced degree;
- make a buffer;
- understand papers that used molar concentrations.




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  #5  
Old 01-21-2008, 06:01 PM
Pow Joshi
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Default Calculating Protein Concentration

On 21/01/2008, StewJW <[Only registered users see links. ]> wrote:



however, it is a matter of common sense to do exactly that ....

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  #6  
Old 01-24-2008, 08:47 PM
Jose de las Heras
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Default Calculating Protein Concentration


"StewJW" <[Only registered users see links. ]> wrote in message
news:[Only registered users see links. ]...

and once, I had an argument with a PI because her primer concentrations were
in pmol/ul and mine were in uM, and I told her they were the same thing...
and she argued that how could they be the same thing.

A PI!

Go figure.

Jose


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