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RNase removal

RNase removal - Protocols and Methods Forum

RNase removal - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 01-08-2008, 07:22 PM
Pow Joshi
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Default RNase removal



On 08/01/2008, Darius Mazeika <[Only registered users see links. ].lt> wrote:



you could use a strategy where you could use a reducing agent like DTT in
your sample preparation. This would cleave the disulphides in
RNases...however, the caveat is that it will reduce every other protein as
well, and especially the ove you are interested in (should it have
disulphide linkages).

best
Pow

I would be greatful for any suggestion.
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