Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


RNase removal

RNase removal - Protocols and Methods Forum

RNase removal - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-08-2008, 03:29 PM
Darius Mazeika
Guest
 
Posts: n/a
Default RNase removal



Hello,

I have a problem and I need help because I don't know what to do. I need
to purify a protein from Tobacco leaves but i can't get rid of RNase
contamination. I tried various chromatography media but it doesn't help.
Traces of RNase are detected. It can't be from my chromatography system or
buffers because I didn't detect any RNase activity. Do you know if APUP
sorbents or media with GDP groups might help?

I would be greatful for any suggestion.
Thanks
Darius

Reply With Quote
Reply

Tags
removal , rnase


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNase A in Qiagen buffer P1 S. Schoenfeld Protocols and Methods Forum 2 10-03-2012 03:40 PM
RNase Zap (Invitrogen) and RNase Away (Molecular Bioproducts) Igor Sagdeev Protocols and Methods Forum 10 02-19-2009 09:39 AM
RNase removal Pow Joshi Protocols and Methods Forum 0 01-08-2008 07:22 PM
RNase away Jayakumar, R Protocols and Methods Forum 0 03-01-2006 03:49 AM


All times are GMT. The time now is 08:55 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11117 seconds with 16 queries