Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Protein/protein-crosslinkers

Protein/protein-crosslinkers - Protocols and Methods Forum

Protein/protein-crosslinkers - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-21-2007, 03:40 PM
Peter Henriksen
Guest
 
Posts: n/a
Default Protein/protein-crosslinkers



I am trying to design an experiment in which I will need to make protein-protein crosslinks but not DNA-protein crosslinks in living cells.

In principal I think this should be possible using a crosslinker that will crosslink between two carboxylic groups but I haven't been able to find such a crosslinker so far.

If anyone has knowledge of a crosslinker with the above properties I would be thrilled to know about it.

Thanks

Peter Henriksen




Reply With Quote
  #2  
Old 11-26-2007, 12:03 PM
Dr Engelbert Buxbaum
Guest
 
Posts: n/a
Default Protein/protein-crosslinkers

Am 21.11.2007, 11:40 Uhr, schrieb Peter Henriksen <[Only registered users see links. ].dk>:


The most simple chemistry to use would be those reagents that cross-link
SH groups.

The best source for info is probably the Molecular Probes catalogue
[Only registered users see links. ]. If that is insufficient, try

@book{Lun-05,
AUTHOR= {R.L. Lundblad},
TITLE= {Chemical reagents for protein modification},
YEAR= {2005},
PUBLISHER= {CRC Press},
ADDRESS= {Boca Raton},
EDITION= {3},
ISBN= {0-8493-1983-8},
PRICE= {129.95 US\$},
LANGUAGE= {engl}
}
Reply With Quote
Reply


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 01:06 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11220 seconds with 15 queries