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96-well plate DNA extraction kits

96-well plate DNA extraction kits - Protocols and Methods Forum

96-well plate DNA extraction kits - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 11-11-2007, 01:48 PM
Caroline Thompson
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Default 96-well plate DNA extraction kits



Hi Guys,

I am usually loathe to resort to commercial kit methods for DNA
extraction, but working with moulluscs and local

prohibitions on phenol/chloroform, it's maybe time to start.



So far I have been using Qiagen spin column kits very successfully, but
I need to move to a 96-well plate format.



Are there any 96-well plate extraction kits available that can be spun
in Sigma 2-16K or 3K15 centrifuges with 11123 / 11223

rotors and 13223 buckets?

Eg Invitrogen kits can indeed be spun at the relatively slow speeds on
these centrifuges (approx 2250g) - but the brochure

suggests a plate height clearance of 5.75cm, and I have no idea where
that is measured from, the ends of the bucket,

the sides of the bucket?

Any ideas?



Caroline



Caroline Thompson

Conservation & Restoration Group

FRS Freshwater Laboratory

Faskally, Pitlochry, Perthshire PH16 5LB

direct 01224 294462

switch 01796 472060



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  #2  
Old 11-13-2007, 02:59 PM
StewJW
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Default 96-well plate DNA extraction kits

These plates are standard SBS format and require a bucket depth of
5.75cm, therefore measure from the base of the bucket, up the side, to
the top brim of the bucket. If you need any further assistance you can
email: [Only registered users see links. ]
Best,
Stewart


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  #3  
Old 11-13-2007, 03:03 PM
StewJW
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Default 96-well plate DNA extraction kits

I assume your refering to Invitrogen's PureLinkô 96 Genomic DNA Kit,
PureLinkô 96 Genomic DNA Kit K1821-04 4 ◊ 96 preps. These plates
can also be used with a vacuum manifold.

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