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Acid Phenol/chloroform

Acid Phenol/chloroform - Protocols and Methods Forum

Acid Phenol/chloroform - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 11-06-2007, 08:10 PM
Ed Siefker
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Default Acid Phenol/chloroform



I got a kit from Ambion to do some RACE, this kit requires acid
phenol/chloroform but doesn't tell me what the composition of this is.
I called Ambion and they didn't know. I couldn't find a recipe in
Maniatis either.

I have a brand new bottle of phenol ph 6.7. What do I need to add to
make "acid phenol/chloroform"? Thanks.
-Ed

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  #2  
Old 11-07-2007, 06:12 PM
Nick Theodorakis
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Default Acid Phenol/chloroform

On Nov 6, 3:10 pm, Ed Siefker <[Only registered users see links. ]> wrote:

Water-saturated phenol is "acid phenol (phenol has a weakly ionizable
hydrogen)." What you have is likely buffered.

Start with solid phenol crystals, and saturate it with good water (you
might need to do it a couple of times). Standard safety disclaimers
apply (wear gloves and eye and body protection, etc.).

Nick

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  #3  
Old 11-08-2007, 09:07 AM
Christian Praetorius
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Default Acid Phenol/chloroform

Nick Theodorakis <[Only registered users see links. ]> wrote:


Since phenol is one of the really nasty chemicals in the molecular
biology lab, I would rather buy the ready-to-use substance.

Christian

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