RT-PCR Troubleshooting - HELP!

hi,
Iam Miss.B.Sarovar,research scholar in the virology
department working on ssRNA plant viruses.I would like
to explain my problem as:i collected the sample,done
rna extraction and pcr too.Initially i got positive
results for the above.But later when i want to
optomize the expt or to do the same iam not getting
the same.for this i checked my work book and analysed
in many ways.when iam doing the expt for the second
time iam getting streak in tne specified region.hope i
may get the answer at the earliest.
thanking you
sarovar


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