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-   -   RT-PCR Troubleshooting - HELP! (http://www.molecularstation.com/forum/protocols-methods-forum/19530-rt-pcr-troubleshooting-help.html)

bheemathati sarovar 11-01-2007 05:45 AM

RT-PCR Troubleshooting - HELP!
 
hi,
Iam Miss.B.Sarovar,research scholar in the virology
department working on ssRNA plant viruses.I would like
to explain my problem as:i collected the sample,done
rna extraction and pcr too.Initially i got positive
results for the above.But later when i want to
optomize the expt or to do the same iam not getting
the same.for this i checked my work book and analysed
in many ways.when iam doing the expt for the second
time iam getting streak in tne specified region.hope i
may get the answer at the earliest.
thanking you
sarovar


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