protein DNA crosslinked complex purification

Hi, Guys:

I am recently doing some UV crosslink between the protein and DNA.(Histone protein and DNA)
Due to our small amount sample about 50 ul and low percentage of successful crosslink. Is there any method to isolate the crosslinked sample out from the DNA with no protein crosslinked.

Your suggestions will be great helpful.

Thanks a lot!

Qiang,

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qiang fu <[Only registered users see links. ]> wrote:

Could you run an EMSA on a preparative scale?
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--
Kaj

If the DNA is Short enough to see a gel shift, do a gel extraction.

[Only registered users see links. ]lid wrote:
-------------------------------------------

Yuval Ebenstein
Department of Chemistry and Biochemistry, UCLA
Young Hall-2002, 607 Charles E. Young Drive East
Los Angeles, CA 90095-1569
Ph: (310) 794-6685; Fax: (310) 267-4672

how about using formaldehyde as used in ChIP assay? directly add
formaldehyde to cultured cells at final 1%, and incubate for 10 min.

Dr Zhonglin Chai, PhD
Head
Cell Proliferation & Fibrosis In Diabetic Complications Laboratory
Diabetes & Metabolism Division
Baker Heart Research Institute
75 Commercial Rd, MELBOURNE
VIC 3004, AUSTRALIA

Tel: +61 3 8532 1231
Fax: +61 3 8532 1100

Post Address:
P.O. Box 6492
St. Kilda Road
Central Melbourne
VIC 8008, AUSTRALIA

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of Yuval
Ebenstein
Sent: Tuesday, 23 October 2007 3:04 AM
To: [Only registered users see links. ]lid; [Only registered users see links. ]
Subject: Re: Re: protein DNA crosslinked complex purification

If the DNA is Short enough to see a gel shift, do a gel extraction.

[Only registered users see links. ]lid wrote:
DNA.(Histone protein and DNA)
successful crosslink. Is there any method to isolate the crosslinked
sample out from the DNA with no protein crosslinked.
-------------------------------------------

Yuval Ebenstein
Department of Chemistry and Biochemistry, UCLA Young Hall-2002, 607
Charles E. Young Drive East Los Angeles, CA 90095-1569
Ph: (310) 794-6685; Fax: (310) 267-4672

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