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Oligos labelling

Oligos labelling - Protocols and Methods Forum

Oligos labelling - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-09-2007, 01:32 PM
3pi
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Default Oligos labelling



Hello all,

The task i'm facing is labelling oligonucleotides with biotin-dutp by
reverse-transcription on their mRNA targets.
Is there anyone who has some experience in it? I'm still having some
poor results, and often non-specific labelling occurs.

Thanks alot in advance.

Patrick.

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