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Polysome Profile HeLa

Polysome Profile HeLa - Protocols and Methods Forum

Polysome Profile HeLa - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 09-08-2007, 02:12 AM
arnigambe@gmail.com
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Default Polysome Profile HeLa



Hi everyone! I'm currently working on HeLa cells and I want to create
a polysome profile of HeLa extracts. I performed the experiment twice
but I could only get a series of +3044's followed by a series of 0+ 's
in the readings. Can anyone please tell me what's going on? Thanks! I
appreciate all help.

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Old 09-08-2007, 07:39 PM
Nick Theodorakis
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Default Polysome Profile HeLa

On Sep 7, 10:12 pm, [Only registered users see links. ] wrote:

I used to run polysomes ages ago, including some from with HeLa cells
(see: Mol Cell Biol. 1987 December; 7(12): 4357-4368, for HeLa; also J
Biol Chem. 1999 Apr 23;274(17):12081-6 for other cells). It's going to
hard to tell what's wrong until you give us some details on what you
are doing.

Nick


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