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RE:protein concentration and desalting

RE:protein concentration and desalting - Protocols and Methods Forum

RE:protein concentration and desalting - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-07-2007, 11:22 PM
Ozan Aygun
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Default RE:protein concentration and desalting



Hi,

I would suggest you to use Strataclean beads from the
Stratagene. For a little volume you will need very
little beads. You can concentrate proteins on these
beads while desalting them. I used this to analyze a
protein purification that was present in 2 M salt
condition in the eluate. After using these beads I was
able to load the concentrated proteins into SDS-PAGE
without any obvious precipitation. Elution to solution
by a low pH buffer should also be possible.

Some new generation membrane-based filters can aslo
help you. (You should check centricon and similar
companies' products)

Moreover, if your sample volume is ~2-3ml there are
very good dialysis systems from the Pierce which you
can desalt your sample before concentrating in
relatively mild conditions.

I hope these may be any help, good luck !

Ozan


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